Additional Selective and Differential Cell Culture Media
NEOGEN Harlequin Listeria Chromogenic Agar according to Ottaviani & Agosti, 500 g
Listeria Chromogenic Agar (according to the formulation of Ottaviani and Agosti) is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials as described in ISO 11290-1:2017. This medium is not intended for use in the diagnosis of disease or other conditions in humans.Lithium chloride in the base medium and supplementary antimicrobial compounds Ceftazidime, Polymyxin, Nalidixic acid and Cycloheximide provide the medium’s selectivity. Chromogenic activity is as a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. and to a few strains of Enterococci and Bacilli.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-listeria-chromogenic-agar-ottaviani-and-agosti
NEOGEN m-Enterococcus Agar, 500 g
m-Enterococcus Agar is used for the selective isolation and enumeration of enterococci by membrane filtration in a laboratory setting. m-Enterococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.m-Enterococcus Agar was first described by Slanetz et al. for the enumeration of enterococci by the membrane filtration technique. In 1957, Slanetz and Bartley modified this medium by adding triphenyl tetrazolium chloride (TTC). Increased recovery and larger colonies were obtained by incubating the inoculated membranes on the agar surface instead of on pads saturated with liquid medium. The membrane filtration method is simple to perform, does not require confirmation, and permits a direct count of enterococci in 48 hours. m-Enterococcus Agar is also referred to as m-Azide Agar.For more information visit https://www.neogen.com/solutions/Microbiology/m-enterococcus-agar
NEOGEN Lactobacilli MRS Agar, 500 g
Lactobacilli MRS Agar is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.MRS Agar is a medium for the cultivation and enumeration of Lactobacillus spp. Originally developed in 1960 by de Man, Rogosa & Sharpe, the medium is suitable for most lactic acid bacteria and is intended as a substitute for Tomato Juice Agar. When acidified to pH 5.4 MRS Agar can be used to enumerate Lactobacillus bulgaricus in yogurts.Nutrition is provided by a mixture of carefully selected peptones, glucose, beef & yeast extracts while Tween 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli while inhibiting a number of other microorganism groups.For more information visit https://www.neogen.com/solutions/Microbiology/lactobacilli-mrs-agar
NEOGEN LESS Plus Medium Irradiated, 500 g
Listeria monocytogenes, described first in 1926 by Murray, Webb and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicates the principle route of transmission is via the consumption of foods contaminated with Listeria monocytogenes. Implicated vehicles of transmission include meat, eggs, chicken, vegetables, and dairy products. Listeria spp. are ubiquitous in nature, present in a wide range of unprocessed foods and in soil, sewage, and river water. Certain strains of Listeria spp. are able to survive the manufacturing and ripening processes in dairy products.For more information visit https://www.neogen.com/solutions/Microbiology/less-plus-medium
NEOGEN Nutrient Broth No. 2, 500 g
Nutrient Both No. 2 is used for the cultivation of fastidious bacteria, and forms the base for Preston Broth according to ISO 10272-1:2017 for the cultivation of Campylobacter spp. This media is not intended for use in the diagnosis of disease or other conditions in humans. This broth can also be used as the suspending medium for cooked meat granules for the cultivation of anaerobic organisms.For more information visit https://www.neogen.com/solutions/Microbiology/nutrient-broth-number-2
NEOGEN Modified Giolitti & Cantoni Broth, 500 g
Modified Giolitti and Cantoni Broth (ISO) is used for the detection and enumeration of coagulase- positive staphylococci from food and animal feeding stuffs using the Most Probable Number (MPN) technique according to ISO 6888-3:2003. This medium is not intended for use in the diagnosis of disease or other conditions in humans.Originally described by Giolitti and Cantoni as a medium for the enrichment of staphylococci from foodstuffs, Mossel later applied the medium to use with samples from dried milk and infant food. Optimized for use in samples where staphylococci may be stressed and/or in low numbers, growth of the target organisms is promoted by sodium pyruvate, Glycine and the high concentration of mannitol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-giolitti-and-cantoni-broth
NEOGEN Tryptone Soy Broth, 500 g
Tryptone Soy Broth (TSB) is a general-purpose broth which will support the growth of a wide range of micro-organisms and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean- Casein Digest Broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry. For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-soy-broth
NEOGEN Modified Buffered Peptone Water with Pyruvate, 500 g
Modified Buffered Peptone Water with Pyruvate (mBPWp) is used for the isolation of Enterohemorrhagic E. coli (EHEC) in a laboratory setting. Modified Buffered Peptone Water with Pyruvate (mBPWp) is not intended for use in the diagnosis of disease or other conditions in humans. Enterohemorrhagic E. coli (EHEC) are recognized as the primary cause of hemorrhagic colitis (HC) or bloody diarrhea. This infection can also lead to hemolytic uremic syndrome (HUS). Enterohemorrhagic infections are usually food or water borne and have been implicated in undercooked ground beef, raw milk, lunchmeat, water, unpasteurized apple cider, sprouts and vegetables. Modified Buffered Peptone Water with Pyruvate is recommended in the sample preparation for the screening method following FDA/BAM Enterohemorrhagic E. coli (EHEC) Protocol.For more information visit https://www.neogen.com/solutions/Microbiology/modified-buffered-peptone-water-with-pyruvate
NEOGEN Sodium Chloride (Bacteriological), 500 g
Sodium Chloride is a salt often used when preparing microbiological culture media in a laboratory setting. Sodium Chloride is not intended for use in the diagnosis of disease or other conditions in humans. When added to culture media Sodium Chloride allows for adjustments in osmotic pressure.For more information visit https://www.neogen.com/solutions/Microbiology/sodium-chloride-bacteriological
NEOGEN Violet Red Bile Agar with MUG (VRBA-MUG), 500 g
Violet Red Bile Agar with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli. Violet Red Bile Agar with MUG is not intended for use in the diagnosis of disease or other conditions in humans. Violet Red Bile Agar W/ MUG is specified in many procedures to enumerate coliforms in food and dairy products. In 1982, Feng and Hartman developed a rapid fluorogenic assay for Escherichia coli by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG) into Lauryl Tryptose Broth. Similarly, incorporating MUG into Violet Red Bile Agar permits the detection of E. coli among the coliform colonies. Standard methods procedures specify Violet Red Bile Agar with MUG for detecting E. coli in food and dairy products by fluorescence. This medium is often abbreviated as VRBA w/ MUG.For more information visit https://www.neogen.com/solutions/Microbiology/violet-red-bile-agar-with-mug
NEOGEN Perfringens Agar Base (TSC), 500 g
This item has a minimum order quantity of 2 per supplier requirements
Perfringens Agar Base (TSC) is used with selective and differential supplements for the recovery of Clostridium perfringens, and is not intended for use in the diagnosis of disease or other conditions in humans. Formulated according to ISO 14189:2013, Perfringens Agar Base (TSC) is a nutritious base medium used in the preparation of SFP Agar and TSC Agar. Depending upon the formula, supplements are added to increase the selectivity of the medium. Shahidi-Ferguson Perfringens (SFP) Agar is based on the formula by Shahidi and Ferguson and incorporates the supplements, Polymyxin B Sulfate and Kanamycin. Tryptose Sulphite Cycloserine (TSC) Agar is developed with the same base as SFP Agar, with the addition of Cycloserine. If desired, Egg Yolk Emulsion can be added to either formula.For more information visit https://www.neogen.com/solutions/Microbiology/perfringens-agar-base
NEOGEN Buffered Peptone Water (BPW), 500 g
Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans. Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods. For more information visit https://www.neogen.com/solutions/Microbiology/buffered-peptone-water
NEOGEN Rappaport Vassiliadis Salmonella Enrichment Broth, 500 g
Rappaport-Vassiliadis Salmonella Enrichment Broth is used for the enrichment and selective isolation of Salmonella spp. Conforms to Harmonized USP/EP/JP Requirements. Rappaport-Vassiliadis Salmonella Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans. A medium recommended by the Harmonized Pharmacopoeia for the enrichment and selective isolation of Salmonella spp.from non-sterile samples. Malachite green and magnesium chloride act as selective agents, combined with high osmotic pressure and low pH to effectively inhibit non-target competitive organisms. Soya peptone provides a source of nitrogen and potassium phosphate acts as a buffer. Some Salmonella (S. typhi and S. enteritidis) are known to be sensitive to malachite green and as such may fail to grow.For more information visit https://www.neogen.com/solutions/Microbiology/rappaport-vassiliadis-salmonella-enrichment-broth
NEOGEN Agar No. 4 - Plant Tissue Culture Grade, 500 g
Agar No.4 has been selected specifically for use as a gelling agent in plant tissue culture techniques, and is not intended for use in the diagnosis of disease or other conditions in humans.Agar No.4 is selected primarily on gel strength, a parameter of particular importance for plant tissue culture techniques, and then tested to ensure it meets the parameters set by a major Horticultural company. The agar contains no nutrients for plant growth and is designed to be incorporated into classical formulations such as Murashige and Skoog, as well as customer’s own formulations.For more information visit https://www.neogen.com/solutions/Microbiology/agar-number-4-plant-tissue-culture-grade
NEOGEN Bismuth Sulphite Agar, 500 g
Bismuth Sulfite Agar is used for the selective isolation of Salmonella spp. In a laboratory setting. Bismuth Sulfite Agar is not intended for use in the diagnosis of disease or other conditions in humans and conforms to FDA/BAM formulation. Salmonellosis can result from consumption of raw, undercooked, or improperly processed foods contaminated with Salmonella. U. S. federal guidelines require various poultry products to be routinely monitored before distribution for human consumption. Bismuth Sulfite Agar is a modification of Wilson and Blair formula. The typhoid organism grows luxuriantly on the medium, forming characteristic black colonies. Gram-positive bacteria and coliforms are inhibited on Bismuth Sulfite Agar. The inhibitory action of Bismuth Sulfite Agar permits the use of a large inoculum, increasing the possibility of recovering pathogens that may be present in small numbers.For more information visit https://www.neogen.com/solutions/Microbiology/bismuth-sulfite-agar